It is used for carbiohydrate fermentation studies and for studies of haemolytic activity of Clostridia, Streptococci and other organisms. Casein enzymic hydrolysate is the only source of nutrient present. Other peptones, rich in carbohydrates like yeast extract or papaic digest of soyabean meal are not used in this medium as this medium favours distinct haemolytic reactions. Addition of sodium thioglycollate and sodium formaldehyde sulphoxylate create conditions necessary for cultivation of anaerobic bacteria which is indicated by methylene blue dye present in the medium.
Application: Used For Carbohydrate Fermentation Studies & For Studies of Haemolytic Activity of Clostridia, Streptococci & Other Organisms
- Appearance of powder: cream coloured, may have slightly greenish tinge, homogeneous, free flowing powder
- Gelling: firm, comparable with 1.5% agar gel
- Colour and clarity: yellow coloured, clear gel forms in petri plates
- Reaction of 3.8% w/v aqueous solution is pH 7.2 ± 0.2 at 25°C
- Cultural characteristics observed after an incubation at 35-37°C for 18 - 48 hours under anaerobic condition