IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli.
IPTG is a galactose analogue not recognized by β-galactosidase. It used as a Lac operon inducer in bacteria. At 1mM concentration, IPTG induces the β-galactosidase gene expression in bacteria, then it allows the selection of Lac+ recombinant bacteria in X-Gal containing medium. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Grade: Genomic Grade
Assay: > 99.0 % (HPLC)
Key Applications: Inducer of the operon in E. coli.
Application Areas: Gene Expression; Transformation; Cloning & Expression
Research Areas: Protein Trafficking; Cell Viability & Cytotoxicity
Product Type: Reagents
Melting Point: 110°C (Lit.)
Optical Rotation: -28.9° to -32° (c=1, water)
Presentation: White Powder
Half life: 2 years
Detection Method: HPLC, GC
Water Content: <1.0%
Dioxane Content: <0.0001%
Solubility: 10 g/L in water.
Storage & Handling: Store at 0°C.
Certifications: RNAse, DNAse, Protease free