Chromatography is the oldest documented technique to separate chemical substances. In this activity, students will perform paper chromatography on three individual dyes and a mixture of dyes to determine if all three dyes are in the mixture. Students will understand not only the components of a chromatography system but also why different substances move at different rates within the system. The kit contains enough materials for 15 groups. Teacher’s Manual and Student Study Guide copymasters are included. Aligned to the Next Generation Science Standards (NGSS)* Disciplinary Core Ideas: PS1.A; PS1.B Performance Expectations: MS-PS1-2; HS-PS1-3 Cross Cutting Concepts: Patterns Engineering Practices: Analyzing and Interpreting Data; Planning and Carrying Out Investigations Kit Includes: 1 Chromatography Dye Set containing: 1 x 0.5mL Crystal Violet 1 x 0.5mL Safranin O 1 x 0.5mL Toluidine Blue 1 x 0.5mL Chromatography Mixture 5 x 30mL Ethanol, 95% 16 Filter Paper Sheets 4 Capillary Tubes DOT Info: Small quantity exemption 173.4 THIS PACKAGE CONFORMS TO 49 CFR 173.4 for domestic highway or rail transport only *”Next Generation Science Standards” is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.