500cm² gas permeable membrane surface area with 5000 mL media capacity. Closed system with semi-automated liquid handling in conjunction with GatheRex Pump. Removable cap designed for tumor fragment addition. Expand 250 million cells into between 15 to 20 billion cells in about 10 days with NO medium exchange. [GAMMA IRRADIATED. For non-clinical, non-therapeutic, research use only.]