It is mainly used in tissue culture media and biological fluids for separation and purification of large volume samples. The whole set includes membrane diaphragm, a graduated, transparent polystyrene funnel connected to a receiving bottle by a connector. The disposable vacuum filter utilizes a vacuum pump to provide pressure differentials for processing large volumes of tissue culture fluids and other laboratory fluid solutions and the volume of processed samples could be up to several liters. The filtered samples can be stored directly in sterile collection bottles.