NUCLEOSIL® NH2/NH2 RP HPLC Columns

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure

• Wide acceptance as routine packings for very different fields of chromatography
• One of the first spherical silicas used in HPLC
• Developed in the early seventies, it became a world-renowned HPLC packing
• Still found in many analytical and preparative application
• An absolutely reliable choice in HPLC
• The largest variety of modified HPLC silicas available on the market

NUCLEOSIL® NH2 / NH2 RP

Amino modified silica phases for HPLC

• USP L8

Features

• Polar aminopropyl modified silica for reversed phase and normal phase chromatography
• Normal phase chromatography with hexane, dichloromethane or 2-propanol as mobile phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
• Reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
• Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile)


• Pore size 100 Å
• Available particle sizes 3 µm, 5 µm, and 10 µm
• pH stability at 20 °C: 2–8
• Carbon content 3.5 %

Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions

Corresponding NUCLEODUR® phase see NUCLEODUR® NH2 / NH2-RP