Tissue Slice Adapter – 1178J61
The Delta T Tissue Slice Adapter is an accessory designed to support a slice of natural tissue such as bowel, trachea, skin, or other natural tissue at the correct distance relative to the coverslip in the Delta T Dish. The specimen is attached to an aperture so that the specimen is constrained to being flat on a carrier that is Z-axis translatable. This allows the specimen to be adjusted in the Z-axis plane relative to the focal range of the objective. The Delta T System accommodates the fluid, thermal, and optical environment. Therefore, all that needs to be done is to define the tissue on a plane parallel to the coverslip and adjust the position the specimen relative to the objective’s plane of focus.
Artificial Membrane Adapter – 1178J62
The Delta T Artificial Membrane Adapter is an accessory to the Delta T Dish that enables cells grown on the basil surface of an artificial membrane to be directly and easily imaged within a Delta T Dish. The Delta T Membrane Adapter is easily adapted to accommodate many of the artificial membrane culturing products on the market. The device shown supports a Costar Transwell™ membrane. The bottom surface of the membrane is observable on an inverted microscope and adjustable in the “Z” axis to bring the membrane into the plane of focus of the objective. Perfusion ports can be used to perfuse the basil surface of the specimen. Both surfaces of the membrane can be perfused separately with the appropriate perfusion tubes attached to the Hinged Perfusion Adapter. The Delta T Artificial Membrane Adapter is reusable and autoclaveable. When ordering this device, Bioptechs requires a sample of the insert to be used.
Note, there is a simple process of growing cells on the basal surface of the membrane so that the cells can be imaged directly through the cover glass bottomed dish.
Brain Slice Adapter – 1178J63
The Bioptechs Brain Slice adapter is an accessory designed for the observation of temperature controlled specimens that have to be suspended in an aperture and maintained in a 3D perfusable micro-observation environment on an inverted microscope. It has a Z-axis translator to position the specimen within the focus range of the objective and fluid ports to keep the specimen oxygenated with perfusion.
Hinged Perfusion Adapter – 1178J64
The Bioptechs Hinged Perfusion Adapter is an accessory to the Delta T System that provides users with a convenient and inexpensive method of supporting perfusion needles in the Culture Dish while maintaining low-volume perfusion over cells during long term-experiments. Perfusion adapters are sold in pairs. One hinge and needle is used for irrigation. The other is used for aspiration. The level of media in the dish can be can be maintained without overflow with the use of the Delta T Micro Perfusion Pump*. Additional supports can be added to hold gas jets, pH probes, cooling apparatus, or other items which do not require critical positioning.
* Bioptechs recommends the use of the Micro Perfusion Pump with the double tubing set for closely regulated micro-perfusion.
Cooling Ring – 1178J65
The Bioptechs Cooling Ring is an accessory to the Delta T Dish that positions a tubular, stainless steel ring containing a circulated chilled fluid to absorb heat from the specimen. The ring is a physical barrier between chilled fluid passing through the ring and the fluid surrounding the specimen. This cooling ring is made of 304 stainless steel and provides the microscopist with a convenient and inexpensive method of reducing the temperature of specimens in Delta T Culture Dishes. The cooling ring is supported on the stage adapter by a hinge mechanism that translates along with the dish. It is easily flipped out of the way to enable easy exchange of dishes in the stage adapter.
Heated Lid – 1178J66
The Bioptechs Delta T Heated Lid is an accessory to the Delta T Dish System. It is an o-ring sealed stainless steel ring containing an ITO coated glass window that attaches to the top of a Delta T Dish which will provide a condensate free optical surface overtop of the media in the dish. It includes a port through which 5% CO2 or a mixed gas can be introduced to maintain pH if necessary. It is autoclavable, reusable and powered by the Delta T Controller.
Heated Lid with Perfusion – 1178J67
The Bioptechs Delta T Heated Lid is an accessory to the Delta T Dish System. It is an o-ring sealed stainless steel ring containing an ITO coated glass window that attaches to the top of a Delta T Dish which will provide a condensate free optical surface overtop of the media in the dish. It includes a port through which 5% CO2 or a mixed gas can be introduced to maintain pH if necessary and two fluid perfusion ports for perfusion of specimens. It is autoclavable, reusable and powered by the Delta T Controller. Specimens can be perfused by attaching perfusion tubing to the additional ports provided. It is recommended to use the Bioptechs Micro-Perfusion Pump with the dual perfusion tubing for this purpose.
Atmospheric Control Barrier Ring (ACBR) – 1178J69
The Atmospheric Control Barrier Ring (ACBR) is an accessory used to exclude ambient air from the contents of a Delta T Dish when using Water "Dipping" Objectives on Upright Microscopes. The ACBR is made of borosilicate glass, autoclaveable and made specifically to fit the lower lens tube of water "dipping" lenses. It is placed around the barrel of the lens to reduce ambient contamination and evaporation, thereby increasing humidity above the specimen and helping to retain the pH during long-term time-lapse imaging. An intermediate adapter ring is also available that is placed under the ACBR and fastens to the top of the dish to facilitate perfusion and/or the introduction of gas.
Non-Perfusable Coverglass Lid – 1178J70, Perfusable Coverglass Lid – 1178J71
The Coverglass Lid is an accessory to the Delta T Culture Dish System that is used when using transmitted, contrast enhancing modes of microscopy. It creates an optical surface above the specimen in the dish thereby preventing fluid motion at the air to liquid interface above the cells that causes the contrast of the image to change from image to image. Therefore, when acquiring a series of images in a contrast enhancing mode of microscopy, all images in the series will have a uniform contrast. The Coverglass Lid fits loosely on the Delta T Culture Dish and supports a 0.5mm x 22mm coverglass in the center of the field 2.5mm above the specimen. The Coverglass Lid is reusable and helps the Delta T bridge the gap between an open dish and a closed system environment. The Coverglass Lid is made of 304 stainless steel, is autoclaveable, and available with or without perfusion. The Cover-glass Lid and/or the Heated Lid are nearly essential for multi-user facilities.
Media Depth Reducer – 1178J72
The Media Depth Reducer is an accessory for Delta T Dishes that rest on top of the dish and supports a hollow cylinder with a cover glass bottom that is lowered into the dish displacing the media in the dish immediately above the specimen. This reduces the depth of media above the specimen plane. Reducing this volume may be desirable when the media contributes to background fluorescence providing better contrast to the specimen, often used for yeast. The Media Depth Reducer is composed of a 0.5mm thick coverglass window mounted in a 22mm diameter stainless steel cylinder, which slides into a Delrin threaded receiver that is adjustable in the Z-axis by rotation of a metal outer ring that rests on the top of the dish.
Coverslip Weight – 1178J74, Coverslip Spacer – 1178J75, Coverslips #1.5 – 1178J73
The Coverslip weight is an accessory for Delta T Dishes that enables live cells grown on ordinary 22mm round coverslips to be observed on an upright microscope. It enables cells to rest in a temperature controlled media environment compatible with the use of high or low N.A. objectives on an upright scope. The Dish provides the temperature control. An autoclavable glass spacer ring provides a media filled well and a surface on which the 22mm round coverslip and coverslip weight is placed. Using this method, you can grow cells on inexpensive 22mm coverslips, then observe them on upright microscopes in a high numeric aperture compatible, temperature controlled, fluid environment with conventional objectives on an upright microscope. This method can reduce some of the workload from other live-cell inverted scopes in a facility. This technique provides a cost effective solution for live-cell imaging especially when combined with a time-lapse camera.