Trypsin inhibitor from Glycine max (soybean), solution, sterile-filtered, suitable for, suitable for cell culture

Synonyms: SBTI

CAS Number: 9035-81-8

MDL No.: MFCD00082103

Storage: -20C

UNSPSC Code: 12352200

Application: Optimized for passage of endothelial cell cultures.Trypsin inhibitor from Glycine max (soybean) was used for the following applications:. Filteration and seeding of dispersed acini. Relative quantification of phospho-tau and immunoblotting analysis. Subcloning and transformation of VIL-4 and IL-4 cDNA, and protein expression and purification (trypsin inhibitor form glycine max is used as positive control).

Biochem/physiol Actions: This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Unit Definition: One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 C.

Preparation Note: The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8 C. Solutions are stable in frozen aliquots at -20C.

Preparation Note: Prepared in Dulbecco's Phosphate Buffered Saline without calcium and magnesium.

Other Notes: Contains chromatographically purified soybean trypsin inhibitor and iron supplemented calf serum.

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