Interleukin-6 from rat, IL-6, recombinant, expressed in E. coli, buffered aqueous solution, cell culture tested

Synonyms: Interleukin-6 from rat

MDL No.: MFCD01323739

Purity: >97% (SDS-PAGE)

Storage: -70C

UNSPSC Code: 12352200

Biochem/physiol Actions: Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-alpha in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-beta-2 (IFN-beta2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form: Supplied as a 0.2 mum filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Analysis Note: The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Preparation Note: Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.