Chemical structure: aminoglycoside
Synonyms: Antibiotic G418
Molecular Formula: C20H40N4O10 · 2H2SO4
Molecular Weight: 692.71
Linear Structural Formula: C20H40N4O10 · 2H2SO4
MDL Number: MFCD00058314
Storage: 2-8C
Application: G418 disulfate is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 mug/mL or less while animal cells may require 300-500 mug/mL.
Biochem Physiol Actions: Mode of Action: G418 blocks polypeptide synthesis and protein elongation by inhibiting synthesis at the 70S and 80S ribosomes.Antimicrobial Spectrum: G418 selects for cells stably transfected with an iNOS promoter construct and neomycin resistance gene and shows activity against protozoa and helminthes.
Biochem/physiol Actions: Mode of Action: Blocks polypeptide synthesis and inhibits elongation in the selection and maintenance of eucaryotic cells stably transfected with neomycin resistance gene
Empirical Formula (Hill Notation): C9H13NO