The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized with an in-house control by immunoelectrophoresis. Each antiserum is tested for specificity at a minimum of 80 mg/mL using immunoelectrophoresis.
The antiserum is prepared by hyperimmunizing rabbits with human serum. The antiserum is delipidated and dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Serum is the component of blood plasma without cells and clotting factors. It is used in various assays, research and also as a supplement in cell culture. Anti-human serum antibody can be used in immunoelectrophoresis. Rabbit anti-human serum antibody reacts specifically with normal human serum.
Rabbit Antiserum to Human Serum is used in immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Rabbit Antiserum to Human Serum is the lyophilized powder of rabbit antiserum to human serum and buffer salts.
Key Applications: Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections
Application Areas: Immunoassays
Product Type: Antibodies
Antibody Type: Primary Antibodies
Antibody Category: Polyclonal
Host: Rabbit
Immunogen: Pooled normal human serum
Species Reactivity: It shows reactivity to human serum; cross-reactivity to other species may exist.
Format: Lyophilized
Solubility: Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Storage & Handling: Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.