The total protein is measured using the Biuret procedure, with bovine albumin as the standard; The product has been tested at 60 mg/mL and found negative against the following whole sera at 20-25 mg/mL: Bear, Deer, Hamster, Mouse, Bovine, Dog, Horse, Rabbit, Cat, Goat, Human, Rat, Chicken, Guinea, Pig, Monkey, Swine.
The antiserum is prepared by immunizing goats with sheep serum; antiserum is delipidated and made specific by immunoadsorption with soluble antigens or solid-phase immunoadsorption where appropriate. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
The human serum proteome as a whole reflects changes in the physiological state of the human body. The onset of diseases invariably induces changes in the protein content, perhaps by producing more of one protein, less of another, or by producing a whole new protein. In all of these situations, the changes offer a direct route for diagnosing the disease.
Whole serum polyclonal antibody is used for immunostaining of cell suspensions, acetone-fixed frozen tissue sections, and formalin-fixed, paraffin-embedded tissue sections. This product is intended for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis, capillary tube, and immunodiffusion techniques.
Product is the lyophilized powder of goat antiserum to sheep serum and buffer salts.
Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. If a small amount of precipitate is present, or if preparation appears cloudy, clarify by centrifugation or filtration.
Concentration: Total Protein: 62.6 mg/mL
Suitability: This product is intended for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis, capillary tube, and immunodiffusion techniques.
Key Applications: Immunoelectrophoresis, Immunostaining, Immunodiffusion, capillary tube
Application Areas: Immunoassays; Immunodiffusion; Immunoeletrophoresis
Product Type: Antibodies
Antibody Type: Primary Antibodies
Antibody Category: Polyclonal
Host: Goat
Species Reactivity: Porcine
Format: Lyophilized
Formulation: Lyophilized PBS with 0.05% Sodium Azide
Foreign Activity: The product has been tested at 60 mg/mL and found negative against the following whole sera at 20-25 mg/mL: Bear, Deer, Hamster, Mouse, Bovine, Dog, Horse, Rabbit, Cat, Goat, Human, Rat, Chicken, Guinea, Pig, Monkey, Swine.
NOTES: Ship: cold packs
Storage & Handling: Store product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage, aliquot and freeze reconstituted product at -20°C or lower.