Potassium chloride is a widely used reagent in biochemistry and molecular biology.
Potassium chloride is a salt commonly used in the preparation of phosphate buffered saline and for the extraction and solubilization of proteins. It is a good potassium source. Also used in the hypotonic KCl-propidium iodide protocol for univariate analysis of metaphase chromosomes; dissociation of eukaryotic ribosomes into subunits by high concentrations of potassium (0.3-1.0 M); preparation of mitochondria, provides an ionic osmotic support for those tissues that assume a gelatinous consistency upon homogenization.
Grade: ACS Reagent Grade
Assay: 99.0 - 100.5%
Key Applications: Extraction and solubilization of proteins
Application Areas: Molecular Biology
Product Type: Inorganics
Biochemical Category: Salts
Chemical Class: Inorganics
Density: 1.984 g/cm3 (Lit.)
Boiling Point: ca. 1500°C (Lit.)
Melting Point: ca. 770°C (Lit.)
Presentation: White Crystalline Powder
Format: Crystalline Powder
pH: 5.4 - 8.6 (5% aq soln)
Heavy Metals: (as Pb) ≤5 ppm
Insoluble Matter: ≤0.005%
NOTES: Iodide ≤0.002%. Bromide ≤0.01%, Chlorate and Nitrate ≤0.003%, Phosphate ≤5 ppm, Sulfate ≤0.001%, ,Iron ≤3 ppm ,Calcium ≤0.002% ,Magnesium ≤0.001% , Sodium ≤0.005%
Solubility: Soluble in water (1 g/3 mL - clear, colorless solution), boiling water (1 g/1.8 mL), glycerol (1 g/14 mL), or ethanol (1 g/250 mL); insoluble in ether or acetone; hydrochloric acid, sodium or magnesium chlorides diminish its solubility in water.
Storage & Handling: Room Temperature, desiccate