Specific Activity: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Application:
Xanthine Oxidase has been used to study tyrosine nitration.
Biochem/physiol Actions:
Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.
General description:
Xanthine:oxygen oxidoreductase
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Quality:
Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.
Physical form:
Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8
Preparation Note:
Activator: O2
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.
Sequence:
XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.