Urokinase-type Plasminogen Activator (uPA) is a 52 kDa serine protease which has been implicated in a number of physiological and pathological processes, including tissue remodeling1, angiogenesis2, fibrinolysis and tumor spread. When bound to its cell surface receptor, uPA is converted from the single chain pro-form uPA to the active 2-chain HMW-uPA. uPA has been shown to play a role in basement membrane degradation, via a cascade involving activation of plasminogen and the matrix metalloproteinases3. Inhibitors of uPA have been shown to slow primary tumor growth and metastasis4-7. The CHEMICON uPA Activity Assay Kit provides a quick, efficient and sensitive system for evaluation of uPA activity and for screening of uPA inhibitors. The assay is colorimetric and does not require radioactivity or fluorescence equipment. The assay is sensitive over a range of 0.05-50 units of uPA activity. Test Principle: The CHEMICON uPA Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA. Addition of this substrate to a uPA-containing sample results in a colored product, detectable by its Optical Density at 405nm (OD405). Application: The CHEMICON uPA Assay Kit is ideal for measurement of uPA activity in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of the enzymatic activity of uPA. Each CHEMICON uPA Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA from human urine as a positive control. Duplicate or triplicate samples are suggested. The CHEMICON uPA Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Application: Research CategoryCell Structure
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