Synonyms: Carbamide; Carbonyldiamide
Formula: NH2CONH2
Formula Weight: 60.06
CAS No.: 57-13-6
Density: 1.335 g/mL at 25 °C(lit.)
Application: Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Abs.: A5M/260 nm ≤0.06
Anion Traces: sulfate (SO42-): ≤0.05%
Cation Traces: Zn: ≤0.0005%
Density: 1.335 g/mL at 25°C(lit.)
Gene Information: human ... CA1, CA2
Ign. Residue: ≤0.1%
Impurities: ≤0.1% Insoluble matter
MP: 132-135°C(lit.)
pH: 7.5-9.5 (20°C, 5 M in H2O)
Product Line: BioXtra
Solubility: H2O: soluble5 M at 20°C, clear, colorless