The gel is formed by stable thioether bonds between maleimide groups of PVA (FAST-PVA) and thiol groups on CD cell-degradable crosslinker. Malemide-functionalized PVA is specifically used to decrease the time for gel formation. The PVA polymer does not have cell attachment sites, but it can be customized to conjugate RGD peptide using TrueGel3D RGD peptide (TRUERGD-1EA) prior to the crosslinking step. The CD cell-degradable crosslinker is composed of matrix metalloprotease (MMP)-cleavable peptide (Pro-Leu-Gly-Leu-Trp-Ala), which allows cells to spread and migrate by secreting matrix metalloproteases (MMP1, MMP3, MMP7 and MMP9).
Application: TRUE4-1KT is used for cell migration studies of amoeboid cells including neutrophils, dendritic cells and lymphocytes. It has been preferred to use PVA-based gels over dextran-based gels for long-term culture of cells producing carbohydrate degrading enzymes.