Fluid Thioglycolate Medium is a universal complex medium. It is recommended for sterility testing of pharmaceutical products according to the European and US Pharmacopoeia. Thioglycolate and L-Cystine in the medium reduce the redox potential of the culture medium to create an anaerobic atmosphere. Also, mercury and other heavy metal compounds are inactivated by these agents. The content of agar further reduces a rapid diffusion of oxygen through the medium but may lead to slight turbidity in larger volumes (filled bottles). Resazurin indicates the reduction potential of the medium. An increased concentration of oxygen is indicated by a color change to pink.
Synonyms: Fluid Thioglycollate Medium, Thioglycollate Broth, FTM
Application: Fluid Thioglycolate Medium is used for the isolation and cultivation of fastidious anaerobic as well as for aerobic microorganisms. This is used for sterility control of pharmaceutical products.