SIGMA Trizma® base, Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, =99.9% (titration)

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Synonyms: 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Tris(hydroxymethyl)aminomethane; Trometamol

Molecular Formula: C4H11NO3

Molecular Weight: 121.14

Linear Structural Formula: NH2C(CH2OH)3

MDL Number: MFCD00004679

Purity: >=99.9% (titration)

Melting Point: 167-172 C (lit.)

Application: Trizma has been used:
•As a component in the culture of human embryonic stem (hES) cells on human and mouse feeder cells
•As a component of pre-hybridization buffer in in situ hybridization
•To suspend dried protein before SDS-PAGE protein characterization
•As a component of lysis buffer to wash and lyse cells before immunoprecipitation
•In the extraction and enrichment of membrane proteins for separation by two-dimensional polyacrylamide gel electrophoresis
•In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
•In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Legal Information: Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.