Purity: ‚≥95% (SDS-PAGE)
Specific Activity: >1*10^8 U/mg (>1 x 10⊥8 U/mg, <0.01 ng/ml (hTNF-a, NIBSC, interim standard, 87/650), at least the same specific activity (EC50) compared to the indicated standard is guaranteed.)
Storage: −20°C
UNSPSC Code: 12352207
RIDADR: NONH for all modes of transport
Application:
Tumor Necrosis Factor-α (hTNF-α) • Shows cytolytic and/or cytostatic activity on a variety of transformed cell lines.
• Has activating and growth stimulating activities on a variety of normal cells.
• Has antiviral activity on many cell types in vitro.
• Can cause tumor necrosis of certain tumors in vivo.
Biochem/physiol Actions:
Receptor p55 and p75, belonging to the TNFR gene family, mediate the biological activity of TNF-a, and are co-expressed on the surface of various cell types. The pleiotropic action of TNF-a is initiated by binding to either of the two receptors.
General description:
Tumor necrosis factor a (TNF-a) is a multifunctional cytokine, majorly produced by activated macrophages and is involved in the pathogenesis of many acute and chronic inflammatory condition.
Recombinant, Tumor Necrosis Factor-α, human (hTNF-α), is produced in Pichia pastoris and purfied by standard chromatographic techniques.
Primary structure: One polypeptide chain (157 amino acids), identical to natural hTNF-a.
Other Notes:
For life science research only. Not for use in diagnostic procedures. 1 EU corresponds to 0.1 ng
Quality:
Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
Physical form:
Solution, filtered through 0.2 μm pore size membrane.
Preparation Note:
Working concentration: 1 ng/ml
For complete cell lysis of sensitive cell lines about 1 ng/ml is recommended.
Working solution: Dilute the concentrated hTNF-α solution (10 μg/ml) with PBS or culture medium containing BSA (or HSA), 1 mg/ml (0.1%) or serum 1 to 10%.
Storage conditions (working solution): -15 to -25 °C
It is recommended to store the solution in aliquots at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.
Sequence:
Chain Length 157 AA
Specificity:
Species specificity: human, mouse