Thiobarbituric acid (TBA) reaction has been used to detect lipid oxidation. When lipids are oxidized, malonaldehyde is formed. TBA reacts with malonaldehyde in hot acid to form a red complex of malonaldehyde:TBA (1:2).
Used to quantitate lipopolysaccharides, carrageenan, and sialic acids; used to detect lipid hydroperoxides and lipid oxidation. 2-Thiobarbituric acid (TBA) is used in TBA assay, or TBA test, which has been employed in the determination of autoxidative alterations of fats and oils. Two processes occur in autoxidation, generally: the free radical and the photo-oxidation mechanisms. TBA has been used to determine iota-carrageenan. It has been also used for quantitating sialic acids in a variety of substances. Reagent for determination of N-acetylneuraminic acid.
Purity: ≥98%
Key Applications: Determination of autoxidative alterations of fats and oils, Reagent for determination of N-acetylneuraminic acid, Used to quantitate lipopolysaccharides, carrageenan, and sialic acids
Product Type: Biochemicals
Melting Point: 235-241°C(Lit.)
Extinction Coefficient (E1%): EmM:12.6, 14.8 (0.1 M phosphate buffer, pH 7.0)(Lit.)
Presentation: Tan Powder
Format: Powder
Storage & Handling: Room Temperature, store under nitrogen