A cell-permeable nonpeptidic nicotinoyl hydrazone compound that selectively targets the SH2 domain of STAT5 (IC50 = 47 µM against STAT5b SH2 domain EPO peptide binding activity), while exhibiting much less effect towards the SH2 domain of STAT1, STAT3, or Lck (IC50 >500 µM). Shown to block STAT5/STAT5 DNA binding activity in K562 nuclear extract and inhibit IFN-alpha-stimulated STAT5, but not STAT1 or STAT3, tyrosine phosphorylation in Daudi cells.
Synonyms: STAT5 Inhibitor - CAS 285986-31-4 - Calbiochem; Nʹ-((4-Oxo-4H-chromen-3-yl)methylene)nicotinohydrazide
Molecular Formula: C16H11N3O3
Molecular Weight: 293.28
Linear Structural Formula: C16H11N3O3
Purity: >=97% (HPLC)
Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes: Muller, J., et al. 2008. ChemBioChem9, 723.