Application: When supplemented with 5-20% heat-inactivated fetal bovine serum, Schneider′s medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila melanogaster. The medium has been used for the growth and maintenance of the cell lines originally derived by Schneider from Drosophila embryos as well as the culture of cells from other dipteran species.
SCHNEIDER′S INSECT MEDIUM is one of the cell culture media available from Sigma. The selection of a nutrient medium is strongly influenced by 1] type of cell, 2] type of culture [monolayer, suspension, clonal] and 3] ° of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
General description: Many insect tissue culture media were formulated to mimic the main physico-chemical properties of the body fluid of specific insects. A cursory survey of the formulas of culture media designed for insect tissues reveals great qualitative and quantitative disparities in composition. Different media designed for the same species may exhibit less similarity than do two media designed for insects of different orders. Various media have been devised for the in vitro culture of Drosophila cells and tissues. The most extensively used are Schneider′s medium and Echalier and Ohanessian′s D-22 medium. Drosophila cells have been employed to study a variety of biological processes including genetics, endocrinology, physiology and cell biology as well as recombinant protein expression.
Storage: 2-8°C
Empirical Formula (Hill Notation): C3H6N2S