Storage: 2-8°C
UNSPSC Code: 12352200
RIDADR: NONH for all modes of transport
Application:
Streptavidin-POD has been used for:
• ELISA
• In situ hybridization
• Western blot
It is also used for immunohistochemistry.
General description:
Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Physical form:
Lyophilizate, stabilized
Preparation Note:
Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
• ELISA: 5 to 50 mU/ml
• Immunohistocytochemistry: 250 to 500 mU/ml
• Western blot: 100 to 250 mU/ml
Reconstitution:
Add 1 ml double-distilled water to a final concentration of 500 U/ml.
The reconstituted stock solution is stable at 2 to 8 °C for 6 months.
Do not freeze reconstituted antibody! Do not add sodium azide!
A further dilution of reconstituted stock solution in double-distilled water is stable for approx. one week at 2 to 8 °C.