RealSeq(R) is a novel method for preparing small-RNA sequencing libraries that reduces incorporation bias in Next-Generation-Sequencing (NGS). Most bias stems from sequence-dependent variability in the enzymatic ligation reactions that attach the two adapters to the 3' and 5' ends of the miRNAs/small RNAs during preparation of sequencing libraries. By using a novel single adapter and circularization, RealSeq(R) greatly reduces library preparation bias. This technology solves the problem of commonly used sequencing library preparations that lead to underdetection of many miRNAs.RealSeq(R)-AC is a small RNA library preparation kit compatible with Illumina Next Generation Sequencing (NGS) technology. RealSeq(R)-AC is optimized for RNA input between 1ng and 1000ng of total RNA from tissue or cells.
Application: This kit is for small RNA library preparation for NGS. RNA-Seq for small RNA and miRNA; NGS library generation for small RNA and miRNA; RealSeq(R)-AC is highly efficient, detecting more miRNAs in total RNA samples.Application NoteRealSeq(R) accurately quantifies small RNA sequenced on the Illumina Platform. RealSeq(R)-AC uses ligation of a single adapter producing small RNA libraries with more miRNAs compared to library prep kits using two adapters.
Legal Information: RealSeq is a registered trademark of SomaGenics, Inc.