Synonyms: Pyranose: Oxygen 2-Oxidoreductase
CAS Number: 37250-80-9
MDL No.: MFCD03458318
Storage: -20C
Enzyme Commission (EC) Number: 1.1.3.10 ( BRENDA | IUBMB )
UNSPSC Code: 12352204
Application: Pyranose oxidase (P20) is used for the determination of D-glucose and 1,5-anhydroglucitol in clinical analysis. It is used to study the biotransformations of carbohydrates and is used as an important marker for glycemic control in diabetes patients.
Biochem/physiol Actions: Pyranose oxidase (P2O) catalyzes the oxidation of aldopyranoses at position C-2 to yield the corresponding 2-ketoaldoses. P2O is a homotetrameric protein that contains covalently bound flavin adenine dinucleotide (FAD). The in vivo substrates of P2O are thought to be D-glucose, D-galactose, and D-xylose. They are oxidized to 2-keto-D-glucose (D-arabino-hexos-2-ulose, 2-dehydro-D-glucose), 2-keto-D-galactose (D-lyxo-hexos-2-ulose, 2-dehydro-D-galactose), and 2-keto-D-xylose (D-threopentos-2-ulose, 2-dehydro-D-xylose), respectively. Pyranose oxidase has significant activity with carbohydrates such as, L-sorbose, D-glucono-1,5-lactone, and D-allose. When pyranose oxidase catalyzes the oxidation of aldopyranoses, electrons are transferred to molecular oxygen which results in the formation of hydrogen peroxide.
Unit Definition: One unit produces 1.0 mumol of hydrogen peroxide per minute at 37Â C, pH 7.0.
Other Notes: Contains glutamate
RIDADR: NONH for all modes of transport
WGK Germany: 3