Protein Kinase A Catalytic Subunit beta, Active human, recombinant, expressed in baculovirus infected Sf9 cells, >=80% (SDS-PAGE), buffered aqueous glycerol solution

Synonyms: PKA Catalytic Subunit beta, Active human; PKA-Cbeta); cAMP-dependent protein kinase

MDL No.: MFCD07371063

Purity: >=80% (SDS-PAGE)

Storage: -70C

UNSPSC Code: 12352200

General description: Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

Application: Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 C using a final concentration of 50 muM [32P] ATP.

Biochem/physiol Actions: cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

Physical form: Supplied as a solution of 5 mug in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.