PARP-1 human, recombinant, expressed in E. coli

Synonyms: NAD+ ADP-ribosyltransferase; Poly(ADP-ribose) Polymerase

MDL No.: MFCD01324157

Storage: -20C

Enzyme Commission (EC) Number: 2.4.2.30  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

General description: Poly(ADP-ribose) polymerase 1 (PARP-1) is associated with the inflammation response during atherosclerosis.

Application: PARP-1 is a nuclear enzyme that synthesizes ADP-ribose polymers from NAD+, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. PARP1 has been used in a study to assess racial and tissue-specific cancer risk associated with polymorphism in the PARP1 gene. It has also been used in a study to investigate inhibitors of PARP-1 for potential cancer treatments.

Biochem/physiol Actions: PARP-1, a nuclear enzyme that synthesizes ADP-ribose polymers from NAD, specifically binds Zn2+ and DNA, and recognizes single-strand breaks in DNA. It is involved in base excision repair, both short-patch and long-patch, rejoining DNA strand breaks and plays a role in p53 expression and activation. A high level of basal neuronal DNA damage and PARP activity has been reported in rat brain tissue. PARP-1 was shown to be required for HIV-1 integration into DNA. If PARP-1 is deficient there is no productive HIV-1 infection.

Biochem/physiol Actions: PARP-1 is inactivated by cleavage into a 24kDA and 89kDA fragment by activated caspase-3 or caspase-7. This results in the decreased ability to repair DNA damage and an increase in apoptosis.

Physical form: Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 200 mM NaCl, 1 mM DTT, 0.1% Triton(TM)-X 100, 50 % glycerol, and 0.1 mg/ml BSA.

Unit Definition: One unit will incorporate 10 femptomole of poly(ADP-ribose) from NAD into 5 mug of immobilized histone proteins in 30 minutes at 22 C in a 96 well plate.