Specific Activity: 30 U/mg; ~30 units/mg protein (At 25 °C with BAEE as the substrate.)
UNSPSC Code: 12352204
RIDADR: NONH for all modes of transport
Features and Benefits:
Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals
Application:
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.
General description:
Cysteine endopeptidase
Other Notes:
For life science research only. Not for use in diagnostic procedures.
Physical form:
Suspension, crystalline, nonsterile
Preparation Note:
Working concentration: 0.05 to 0.5 mg/ml
Specificity:
Papain is a cysteine endopeptidase, which hydrolyzes proteins, peptides, amides, and esters of amino acids and peptides, specifically at bonds involving the amino acids Arg, Lys, Glu, His, Gly, and Tyr. Upon prolonged incubation, further bonds are split. It also exhibits esterase and transamidase activity and is used for peptide synthesis.