Components
AEBSF, 104 mM
Aprotinin, 80 µM
Bestatin, 4 mM
E-64, 1.4 mM
Leupeptin, 2 mM
Pepstatin A, 1.5 mM
Application
Use at 1:100 for undiluted blood samples, liver tissue extracts, and CHO cell lysates.
Other Notes
A mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatinA, E-64, bestatin, leupeptin, and aprotinin. Contains no metal chelators.
Quantity
One mL is recommended for the inhibition of proteases extracted from 20 g of bovine liver.
Specificity
Inhibits, serine, cysteine, aspartic proteases, and amidopeptidases.
Physical form
Solution in DMSO