Proteolytic enzymes such as trypsin, collagenase and pronase are commonly used for dispersing tissues and cells. These enzymes, however, often injure the cells, are unstable during incubation, can be heterogeneous and also a source of mycoplasma contamination. Dispase-II is used for the preparation of cells from a wide variety of different tissues and organs. Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro. Dispase is especially suitable for tissue disaggregation and subcultivation procedures since it does not damage cell membranes. PluriSTEM Dispase-II has been validated to work alongside PluriSTEM Human ES/iPS Medium (SCM130) for the culture and passage of human embryonic and induced pluripotent stem cells.Dispase-II enzyme is produced in Bacillus polymyxa.
Synonyms: Dispase, Dispase 2
Application: Research Sub CategoryEnzymes & Biochemistry
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