Phospholipids are a class of lipids, which constitute a major component of cell membranes and lipid bilayers. Phospholipids play important roles in signal transduction. The majority of phospholipids contain one diglyceride or sphingosine, a phosphate group, and one choline. The Phospholipid quantification assay kit provides a simple and highly sensitive method for measuring choline-containing phospholipids in biological samples. This high-throughput assay can be done within only 30 minutes . In this assay, phospholipids (such as lecithin, lysolecithin, and sphingomyelin) are enzymatically hydrolyzed to release choline, which is determined using choline oxidase and an H2O2 specific dye. The readout is either fluorescence or absorbance, allowing maximal flexibility and a large detection range. The linear range is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance). Phosphatidylcholine-containing phospholipids are present in serum at ~0.2-2.5 mM (~50-200 mg/dL).The detection range of the kit is 0.1-0.5 nmol (fluorescence) and 1 to 5 nmol (absorbance).
Synonyms: DDPC; DEPC; DLOPC; DLPC; DMPC; DOPC; DPPC; DSPC; EPC; HEPC; HSPC; LYSOPC; MPPC; MSPC; PC; PMPC; POPC; PSPC; Phosphatidylcholine; SMPC; SOPC; SOPC; lecithin; lysolecithin; sphingomyelin
Storage: -20C
Application:
•Measuring choline-containing phospholipids in biological samples (For example: Serum and Plasma)
•Enables evaluation of drug effects on phospholipids metabolism