PNGase F from Elizabethkingia miricola, buffered aqueous solution

Synonyms: N-Glycosidase F; Peptide N-glycosidase

CAS Number: 83534-39-8

MDL No.: MFCD00131222

Storage: 2-8C

Enzyme Commission (EC) Number: 3.5.1.52  ( BRENDA | IUBMB )

UNSPSC Code: 12352204

Application: Used to deglycosylate protein.

Biochem/physiol Actions: Cleaves an entire glycan from a glycoprotein provided the glycosylated asparagine moiety is substituted on its amino and carboxyl terminus with a polypeptide chain.

Physical form: Supplied as a solution in 20 mM Tris HCl, pH 7.5, 50 mM NaCl and 1 mM EDTA

Unit Definition: One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.