MDL No.: MFCD00164585
Storage: -20C
UNSPSC Code: 12352203
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General description: Dynein is a motor protein that regulates the movement of organelles to the 'minus' end of microtubules. Dyenins consist of two heavy chains and many intermediate and light chains. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement In immunoblotting assays, monoclonal anti-dynein (Heavy Chain) is specific for cytoplasmic dynein heavy chain (440 kDa) using dynein enriched preparations, as well as in crude cell extracts of chicken brain and fibroblasts. Upon cleavage of the heavy chain into two fragments (of approximately 200 kDa by exposure to U.V. light in the presence of ATP and vanadate), it is observed that the antibody reacts exclusively with the lower molecular weight fragment. The product reacts with kinetochores in HeLa chromosomes but does not bind to chromosomal dynein in Chinese hamster ovary cell line. Moreover, the antibody product does not react with Chlamydomonas flagella dynein.
Application: Monoclonal Anti-Dynein (Heavy Chain) antibody is suitable for use in western blot (1:100 using a chick brain extract) and indirect ELISA. The product may also be used for immunoblot, dot blot and immunocytochemistry. Using immunofluorescent techniques, the staining of the spindle with the antibody is clearly visible through diffuse cytoplasmic staining in intact mitotic chicken embryo fibroblasts.
Immunogen: cytoplasmic dynein from chicken embryo brain.
RIDADR: NONH for all modes of transport
WGK Germany: 2
R Codes: 21/22
S Codes: 36/37-60
IOD Codes: Xn