Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.
Synonyms: Anti-TRP53; Anti-Transformation-related protein 53
MDL Number: MFCD00164709
Storage: -20C
Application: Monoclonal Anti-p53 antibody produced in mouse has been used in:
• immunoblotting
•enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
•immunohistochemistry
•immunoprecipitation
Biochem Physiol Actions: p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.