Monoclonal Anti-Tenascin antibody produced in rat, clone MTn-12, ascites fluid

MDL No.: MFCD00164746

Storage: -20C

UNSPSC Code: 12352203

General description: Tenascin is a high molecular weight, multifunctional, extracellular matrix glycoprotein expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM).The tenascin molecule is a disulfide-linked hexamer; depending on species, the molecular weights of the subunits range from 190 to 320 kDa. In the mouse, two major subunits of tenascin with an apparent molecular weight of 210 and 260 kDa have been described. The shorter polypeptide predominates during earlier developmental stages and the larger polypeptide appears later in the embryonic gut and especially in the adult intestine.Human tenascin has 3 subunits of 190, 200 and 220 kDa. Tenascin has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. It was proposed that actively growing, migrating and differentiating epithelial sheets can produce factors that can stimulate tenascin expression in the nearby mesenchyme. Human and chicken tenascin contain an RGD sequence which may function in cell adhesion and it seems likely that tenascin mediates cell attachment through an RGD dependent integrin receptor.

Application: Monoclonal Anti-Mouse Tenascin antibody may be used for the localization of tenascin and to study of the role of tenascin in epithelial-mesenchymal interactions using various immunochemical assays including ELISA, immunoblot, dot blot and immunohistology.

Application: Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunohistochemistry (1 paper)

Immunogen: partially purified mouse tenascin.

Specificity: The antibody localizes mouse tenascin in the supernatant of cultured mouse fibroblasts and tissue extracts. No cross-reactivity with tenascin of other species has been observed. In immunohistological testing of frozen tissue sections of mouse intestine, the antibody labels the core of the villi, but not the epithelial cells.