MDL No.: MFCD00145892
UNSPSC Code: 12352203
General description: Î²-Tubulin belongs to the subfamily of tubulin, which is the major building block of microtubules. Î²-tubulin has a molecular weight of 55kDa. Î²-Tubulin structure is characterized with core of two Î²-sheets enclosed by Î±-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site, and a C-terminal domain that contains the binding surface for molecular motor proteins.Human Î²-tubulin consists of seven isoforms (Î²I (class I), Î²II (class II), Î²III (class III), Î²IVa (class IVa), Î²IVb (class IVb), Î²V (class V), and Î²VI (class VI)).
Application: Monoclonal Anti-Î²-Tubulin antibody produced in mouse has been used in western blotting and fluorescence immunocytochemical staining.
Biochem/physiol Actions: Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations. B2702 peptide binds to Î²-tubulin and inhibits NK cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Î²-1 tubulin also known as class VI or TUBB1, plays a vital role in platelet production, and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
Immunogen: sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes.
Specificity: The antibody recognizes the two major and one of the minor beta-tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and beta-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting.
RIDADR: NONH for all modes of transport
WGK Germany: 3