Monoclonal Anti-beta-COP antibody produced in mouse, clone M3A5, ascites fluid

MDL No.: MFCD00164606

Storage: -20C

UNSPSC Code: 12352203

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General description: The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

Application: Monoclonal Anti-β-COP antibody produced in mouse may be used for the localization of β-COP using immunoprecipitation, immunocytochemistry, immunoblotting. This antibody may be used along with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell.

Biochem/physiol Actions: COPs (coatomer proteins) contain adaptin-like, complex and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

Immunogen: microtubule-associated protein from goose brain.

Specificity: The antibody recognizes an epitope shared by β-COP (110 kDa) found in most tissue culture lines, and by a doublet of brain microtubule-associated protein (MAP2, 270-300 kDa). The antibody stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of Golgi complex and a population of coatomers scattered throughout the cytoplasm) in tissues from different species and cell processes, as well as cell bodies in chicken brain neuronal cells. It has been used for studies on the effects of various agents that influence energy status, disrupt the Golgi complex, or alter the activity of G-proteins or small GTP-binding proteins on the cellular localization of β-COP. The antibody may be used for the immunoaffinity purification of the protein.