Produced by plasma cells and lymphocytes, immunoglobulins (antibodies) are critically involved in immune response, attaching to antigens, and playing a role in their destruction. Immunoglobulins (Ig) can be classified by isotype, classes that differ in function and antigen response due to structure variability. Six major isotypes have been identified in placental mammals: IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA - all found in normal individuals. Immunoglobulin-deficiency disorders, such as autoimmune disease, some GI conditions, and malignancies, are characterized by specific isotype deficiencies or varying concentrations of one or more isotypes. Disease states can range from the absence of one isotype class or subclass to a total deficiency of immunoglobulin classes. MILLIPLEX(R) Mouse Isotyping Bead Panel, measuring mouse IgG subclasses (1, 2a, 2b, 3), IgM, and IgA, has been designed to enable you to measure both classes and subclasses accurately - all in one well. In addition, this panel allows you to choose only those immunoglobulins you need for your experiments. The Luminex(R) xMAP(R) platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume. Panel Type: Immune Response
Application:
•Analytes: IgG1, IgG2a, IgG2b, IgG3, IgA, IgM
•Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant
•Recommended Sample dilution: 1:50,000 diluted plasma or serum, or cell culture supernatant diluted to approximately 1mug/mL Ig well.
•Assay Run Time: One day
•Research Category: Immunology and Inflammation, Isotyping
Legal Information: xMAP is a registered trademark of Luminex Corp