Product Source: LIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moeity with thrombin and purified by HPLC chromatography.
Synonyms: LIF
Purity: >95% (HPLC and SDS-PAGE)
Application: RECOMMENDED QC PROTOCOL M1 Bioassay 1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 100 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar. 2. Add 100 muL of sample or hLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes. 3. Add 100 muL of 5% FCS in isotonic saline to two control slides. 4. Incubate at 37C in fully humidified atmosphere of 10% CO2 in air for 7 days. 5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated). Visit www.esgro-lif.com for additional information Manufactured by CHEMICON International, Inc. LIF is protected under US Patent nos. 5,443,825, 5,750,654 and 6,261,548, European Patent no. 0285 448 and related foreign patents and is not available for resale.
Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany