Storage: -20C
UNSPSC Code: 41106300
Features and Benefits: . Assay results in as little as 33 minutes. Highly efficient and sensitive real-time PCR results. Little/no optimization required
General description: KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl2 and proprietary buffers and stabilizers.
Application: Instrument Compatibility:Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart SYBR Green qPCR ReadyMix does not contain an internal reference dye. Compatible instruments include:. Bio-Rad(R) CFX384(TM). Bio-Rad CFX96(TM). Bio-Rad MiniOpticon(TM). Bio-Rad/MJ Chromo4(TM). Bio-Rad/MJ Opticon 2 . Bio-Rad/MJ Opticon(TM). Cepheid SmartCycler(R). Eppendorf Mastercycler(R) ep realplex . Eppendorf Mastercycler ep realplex2 s . Illumina Eco qPCR . Qiagen/Corbett Rotor-Gene(R) 3000. Qiagen/Corbett Rotor-Gene 6000. Qiagen/Corbett Rotor-Gene Q . Roche LightCycler(R) 480
Application: PCR applications:. Gene expression. DNA quantitation. CHiP
Components: 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, and stabilizerspackaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume
Other Notes: Storage Conditions:KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20C, protected from light. For convenience, it may be stored unfrozen at +2 to +8C for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20C.
RIDADR: NONH for all modes of transport
WGK Germany: 3