KOD One(TM) PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA <10kb. this master mix has greater efficiency and flexibility than conventional pcr enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. in addition to crude samples, it can be used on templates containing uracils (du). the master mix can use primers containing both inosines (di) and du for amplification.this hot start kod polymerase is antibody-inactivated with two types of antibodies that prevent the 5 ->3' polymerase activity and 3'->5' exonuclease activity. Upon heat-activation, the 3'->5' proof-reading ability of the enzyme generates blunt-end PCR products.
Synonyms: Fast Hot-start PCR; High-fidelity DNA polymerase; KOD One(TM) PCR Ready Mix
Storage: -20C
Application:
•Genomic DNA amplification
•cDNA amplification
•Direct PCR
•Colony PCR
•Amplification of NGS libraries
•Site direct gene mutationCompatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.Sample Volume Needed: about 50ng DNA
Legal Information: KOD One is a trademark of Toyobo Co., Ltd.
Other Notes: For R&D use only. Not for drug, household, or other uses.