Recombinant human Interferon-gamma (IFN-gamma) is expressed in human 293 cells as a glycoprotein with a calculated molecular mass of 16 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture. The bioactivity of IFN-gamma expressed in human 293 cells is significantly higher compared to bacterially expressed IFN-gamma.
Synonyms: IFN-gamma
MDL Number: MFCD00131391
Purity: >=98% (SDS-PAGE)
Storage: -20C
Application: It has also been used for cytokine treatment of human islets to evaluate the activation of hypoxia inducible factor 1 a (HIF1a) targets, in vitro.
Biochem Physiol Actions: IFN-? is a dimerized soluble cytokine that is the only member of the type II class of interferons.Interferon-? (IFN-?) plays an essential role in function of virtually all immune cells and both innate and adaptive immune responses. IFN-? exhibits various biological effects, such as antiviral activity, inhibition of cell or tumor growth and promotion of terminal differentiation of B cells into immunoglobulin-producing cells. This cytokine also activates macrophages, increases cytotoxicity of natural killer cells and promotes T cell cytotoxicity. In addition to antiviral activity, recombinant human IFN-? is a potent modulator of immune responses and modifies cellular processes.