The ID3EAL™ miRNA qPCR solution adopts a 3-specific assay priming strategy. The conformational-restricted miRNA-specific RT primer efficiently hybridizes to the mature but not the precursor form of the target miRNA. Specific qPCR forward and reverse primers confer further amplification specificity. Coupled with high performance proprietary ID3EAL RT and qPCR master mixes, robust amplification can be completed in just 2 hours with enhanced signal-to-noise ratio.