Guanosine 5'-[beta,gamma-imido]triphosphate trisodium salt hydrate, >=85% (HPLC), powder

Synonyms: beta:gamma-Imidoguanosine 5'-triphosphate trisodium salt hydrate; 5'-Guanylyl-imidodiphosphate trisodium salt hydrate; GMP-PNP; Gpp(NH)p

CAS Number: 148892-91-5

Formula: C10H14N6Na3O13P3 · xH2O

Formula Weight: 588.14 (anhydrous basis)

Linear Formula: C10H14N6Na3O13P3 · xH2O

MDL No.: MFCD00084770

Purity: >=85% (HPLC)

Storage: -70C

UNSPSC Code: 41106305

Application: Guanosine 5′-[β,γ-imido]triphosphate (GMP-PNP, Gpp(NH)p) is a non-hydrolyzable analog of GTP that binds and irreversibly activates G proteins. Gpp(NH)p is used in a variety of applications that involve GTP binding, including GTP-activation, GTP-inhibition, GTP transport, GTP hydrolysis, and GTP structure stabilization. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, guanosine 5′-[β,γ-imido]triphosphate is often used in studies of protein synthesis. Gpp(NH)p is used to activate ADP-ribosylation factors and to modulate G proteins involved in cell signaling, protein synthesis and other metabolic processes.

Application: Non-hydrolyzable analog of GTP; the preferred GTP analog to activate ADP-ribosylation factor.

Biochem/physiol Actions: Binds and irreversibly activates G proteins. Since a cycle of GTP binding, hydrolysis, and release is required for the initiation of protein translocation across the endoplasmic reticulum, this non-hydrolyzable GTP analog is often used in studies of protein synthesis.