Glutathione Peroxidase from bovine erythrocytes, lyophilized powder, >=300 units/mg protein

Synonyms: GSH-Px; Glutathione:hydrogen-peroxide oxido-reductase

CAS Number: 9013-66-5

MDL No.: MFCD00131195

Storage: -20C

Enzyme Commission (EC) Number: 1.11.1.9  ( BRENDA | IUBMB )

EC Number: 232-749-6

UNSPSC Code: 12352204

Application: Glutathione peroxidase from bovine erythrocytes was used as a positive control in cloning and characterization of full-length cDNAs encoding two glutathione peroxidases (GpXs) from Globodera rostochiensis. It was used for the determination of glutathione peroxidase activity in human milk.

Biochem/physiol Actions: Glutathione peroxidase is an enzyme which reduced lipid hydroperoxides into their corresponding alcohols. It also reduces free hydrogen peroxide in to water. In vivo it is responsible for protecting hemoglobin from oxidative breakdown.

Biochem/physiol Actions: Protects cells against oxidative damage by catalyzing the reduction of hydrogen peroxide in the presence of reducing agent glutathione. In cellular membranes it may induce lipid peroxidation through the reduction of hydrogen peroxide or polyunsaturated fatty acid hydroperoxides.

Physical form: Lyophilized powder containing 25% sucrose and 2.5% dithiothreitol with sodium phosphate buffer salts

Unit Definition: One unit will catalyze the oxidation by H2O2 of 1.0 mumole of reduced glutathione to oxidized glutathione per min at pH 7.0 at 25 C.

Other Notes: Note: At the reported pH optimum of 8.8, we have found the activity to be approx. 10 times that at pH 7.0. However, to remain consistent with literature and avoid complications arising from non-enzymatic oxidation of GSH, our unit is defined at pH 7.0.