Synonym(s): CIG; Cold insoluble globulin
CAS No.: 86088-83-7
Application: Recommended for use as a cell culture substratum at 1-5 μg/cm or 0.5-50 μg/ml. Optimal concentration depends on cell type as well as the application or research objectives.
Physical form: Lyophilized in CAPS buffered saline
Analysis Note: Protein homogeneity is evaluated by immunoelectrophoresis.
Caution: Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
EC No.: 289-149-2
Biochem/physiol Actions: Adhesion glycoprotein of the extracellular matrix isolated from human foreskin fibroblasts maintained in serum-free medium. Exists as a dimer with the A chain and the B chain containing the type III connecting segment (IIIcs) region. Different forms of fibronectin appear to be generated from tissue-specific splicing of fibronectin mRNA, transcribed from a single gene. Multiple domains of fibronectin show binding affinities for collagen, fibrin, heparin and specific cell membrane receptors. The most notable domain, Arg-Gly-Asp (RGD), is recognized by integrins and mediates cell adhesion. Involved in widespread interactions and functions such as the attachment and migration of many cell types, cytoskeletal assembly, tyrosine phosphorylation, and metastasis.
Plasma fibronectin molecular weight (heterodimeric): 450 kDa; Cellular fibronectin: 550 kDa
Differs from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemagglutination.
Reconstitution: Store reconstituted solution in working aliquots at -20°C or lower.
Empirical Formula (Hill Notation): C12H11NO