ROCHE FastStart™ Taq DNA Polymerase, dNTPack

Storage: −20°C

UNSPSC Code: 12352200

Components: FastStart Taq DNA Polymerase, in storage and dilution buffer 5 U/μl; PCR Reaction Buffer, with 20 mM MgCl₂ 10x concentrated; PCR Reaction Buffer, without MgCl₂ 10x concentrated; MgCl₂ Stock Solution 25 mM; GC-RICH Solution 5x concentrated; PCR Nucleotide Mix

RIDADR: NONH for all modes of transport

Features and Benefits:
Volume activity: 5 U/μl
Each dNTPack contains 10 mM additive-free sodium salt nucleotides as a ready-to-use mix.

• Higher specificity, sensitivity, and yield:
Hot start PCR makes PCR setup easier.
• Use robotic setup.
Use this enyzme mix stable for 24 hours at +15 to +25°C.
• Prevent PCR carryover contamination.
Incorporate dUTP and use Uracil-DNA Glycosylase to pretreat PCR master mixes.
• Cost-effective.
Use the convenient premixed solution of PCR grade nucleotides.

Application:
FastStart^™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. FastStart Taq DNA Polymerase is an ideal tool for hot start PCR, because the enzyme remains inactive during PCR setup and prior to the initial denaturation step. It can be applied for PCR:
• Hot start PCR up to 3 kb
• Hot Start RT-PCR up to 3 kb
• Multiplex PCR
• Difficult templates, e.g., secondary structures or GC-rich sequences
• Automated PCR, e.g., handling at room temperatures

For maximum convenience, select the 2x concentrated ready-to-use FastStart PCR Master.

General description:
The enzyme dNTPack comprises FastStart Taq DNA Polymerase and a ready-to-use solution of PCR grade nucleotides. FastStart Taq DNA Polymerase is a versatile enzyme that can be used in a wide variety of applications and on multiple instrument platforms. This modified, thermostable, recombinant Taq DNA polymerase is inactive at temperatures below +75 °C, but is activated by a 2 to 4 minute heat activation step at +95 °C. Since it is inactive at low temperatures, FastStart Taq DNA Polymerase cannot elongate non-specific primer-template hybrids that may form at those temperatures. An optimized PCR buffer system and a GC-RICH Solution to enable the enzyme to handle a wide range of templates are also supplied.

Other Notes:
For life science research only. Not for use in diagnostic procedures.

Hot start protocols improve PCR specificity, sensitivity, and yield. Heat-labile blocking groups make the modified enzyme inactive at +15 to +25°C. No elongation occurs when primers bind nonspecifically. The enzyme is activated by removing the blocking groups at +95°C for 2 to 6 minutes. PCR products with 3′-single A overhangs are produced, ideal for T/A cloning. For PCR carryover prevention, use the PCR Nucleotide Mix^PLUS and Uracil-DNA Glycosidase, heat-labile.

Quality:
Each lot is function-tested using human genomic DNA and primers specific for the 365 bp fragment of human tPA gene, and, a 284 bp fragment of the Apo E gene with 74% GC content. Each lot is also tested for the absence of exo- and endonucleases and nicking activity.
For details please refer to the respective Instruction for Use.

Legal Information:
FastStart is a trademark of Roche