Synonyms: Anti-ddddk; Anti-dykddddk
Storage: -20C
UNSPSC Code: 12352200
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Features and Benefits: . Utilizes highly specific ANTI-FLAG M2 affinity gel. No preliminary steps or calibrations. 3X FLAG(TM) peptide provides efficient and gentle elution through direct competition
General description: Sigma's FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.
Application: Browse additional application references in our FLAG(R) Literature portal.
Other Notes: All prodedures to be performed at 2-8C.
Components: ANTI-FLAG M2 affinity gel 1 mL; FLAG-BAP(TM) control protein 50 mug; 3X FLAG peptide 1 mg
RIDADR: NONH for all modes of transport
WGK Germany: 3
Symbol: GHS05
Signal Word: Danger
Hazard Statements: H315-H318
Precautionary statements: P280-P305 + P351 + P338