MDL No.: MFCD00163600
Storage: 2-8C
UNSPSC Code: 12352203
Application: ExtrAvidin(R) may be used in conjunction with biotin in techniques such as ELISA, RIA, immunohistochemistry, protein blotting, and with DNA probes. It was used to capture biotinylated RNA in a study to understand translation initiation pathway using immobilised mRNA analogues. It was used to bind to biotinylated surface during preparation of substrates for protein assembly.
Biochem/physiol Actions: ExtrAvidin(R) is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells.
Specificity: Binding Activity: at least 10 mug biotin per mg
Other Notes: A modified form of affinity purified egg white avidin.
RIDADR: NONH for all modes of transport
WGK Germany: 3